| 沈建，范刚，杨健.高纯度莱鲍迪甙A的结晶制备工艺[J].轻工机械,2015,33(5): |
| 高纯度莱鲍迪甙A的结晶制备工艺 |
| Crystallization Preparation Technology on High Purity Rebaudioside A from Steviol Glycosides |
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| DOI：IO. 3969/j . issn. 1005 -2895. 2015. 05. 001 |
| 中文关键词: 甜菊糖；莱鲍迪甙A 结晶；纯化 |
| 英文关键词:Stevia glycoside Rebaudioside A crystallization purification |
| 基金项目:国家自然科学基金(NO．51375444) |
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| 中文摘要: |
| 针对现有结晶法制备甜菊糖中存在的结晶步骤复杂、回收率低、结晶周期长等问题，提出了二次结晶法从甜菊糖
粗品中提取高纯度RA的新工艺。同时研究了提取溶剂、固液比、结晶温度、结晶时间等对结晶效果的影响，优化了现有
结晶工艺，简化了结晶步骤。实验表明，结晶法制备高纯度RA的最佳工艺为：一次结晶提取溶剂为无水甲醇(质量分数
>99%)，固液比为1：5g/mL，溶解温度为50℃，结晶时间为2h；二次结晶提取剂为86% -88%的乙醇溶液，固液比1:4
g/ml溶解温度为50℃，结晶时间为16 - 24 h。两次结晶RA总得率为70. 9%，通过本结晶工艺，可制得RA的质量分
数为97. 51%的甜菊糖产品 |
| 英文摘要: |
| A new separation process of high purity RA from Stevia sugar raw material was proposed by using the two
cooling crystallization to solve the problems of the complex crystallization steps, low recovery rate and long period of
crystallization existing in crystallization of stevioside. The effects of key operations. including solvents, solid-to-liquid
ratio, crystallization temperature , crystallization time on the purity of products were studied to optimize the crystallization
condition. It indicates that. the best process of preparing high purity RA is as follows. The first extraction solvent is
anhydrous ethanol (mass fraction > 99% ) . solid-to-liquid ratio of l: 5 g/mL, water-bath temperature of 50 0C . and
crystallization time of 2 hours. The second extraction solvent is of 86% - 88% ethanol. solid-to-liquid ratio of l: 4 g/
mL. water-bath temperature of 50 °C . and crystallization time of 16 - 24 hours. The yield of RA is 70.9% by the two
cooling crystallization. Under such conditions. the Stevia products in which the mass fraction of RA is more than 97%
were obtained. |
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